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antibody microarray assay service kam 1325  (Kinexus Bioinformatics Corporation)

 
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    Kinexus Bioinformatics Corporation antibody microarray assay service kam 1325
    Antibody Microarray Assay Service Kam 1325, supplied by Kinexus Bioinformatics Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody microarray assay service kam 1325/product/Kinexus Bioinformatics Corporation
    Average 90 stars, based on 1 article reviews
    antibody microarray assay service kam 1325 - by Bioz Stars, 2026-03
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    antibody microarray assay service kam 1325  (Kinexus Bioinformatics Corporation)
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    Kinexus antibody <t>microarray.</t> Fibroblast growth factor receptor-like protein 1 (FGFRL1) inducible cells (clone K3F) were cultivated for 32 h in the presence or absence of the inducer doxycycline. Protein extracts were prepared and analyzed on Kinexus antibody microarrays containing more than 650 different antibodies against various signaling proteins. No significant differences were observed between doxycycline-treated and untreated cells (not shown). To verify some of the results, the two protein extracts were separated on SDS polyacrylamide gels, transferred to an immunoblot membrane and probed with antibodies against 18 selected proteins as indicated using a multiscreen apparatus from BioRad. The immunoblot was analyzed with a chemiluminescence imager. No significant differences were observed between doxycycline-induced (lower panel) and uninduced (top panel) cells. The migration positions of molecular weight markers are shown in the left and right margins by short horizontal lines. These lines correspond (from top to bottom) to 250 K, 150 K, 100 K, 75 K, 50 K, 37 K, 25 K, 20 K and 15 K.
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    Kinexus antibody <t>microarray.</t> Fibroblast growth factor receptor-like protein 1 (FGFRL1) inducible cells (clone K3F) were cultivated for 32 h in the presence or absence of the inducer doxycycline. Protein extracts were prepared and analyzed on Kinexus antibody microarrays containing more than 650 different antibodies against various signaling proteins. No significant differences were observed between doxycycline-treated and untreated cells (not shown). To verify some of the results, the two protein extracts were separated on SDS polyacrylamide gels, transferred to an immunoblot membrane and probed with antibodies against 18 selected proteins as indicated using a multiscreen apparatus from BioRad. The immunoblot was analyzed with a chemiluminescence imager. No significant differences were observed between doxycycline-induced (lower panel) and uninduced (top panel) cells. The migration positions of molecular weight markers are shown in the left and right margins by short horizontal lines. These lines correspond (from top to bottom) to 250 K, 150 K, 100 K, 75 K, 50 K, 37 K, 25 K, 20 K and 15 K.
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    Image Search Results


    Downstream signaling hits between cells expressing wild-type PBK and cells expressing p.Gly43Arg PBK (control and treatment) for 24 hours as analyzed by KAM-900P Antibody Microarray.

    Journal: Scientific Reports

    Article Title: A novel loss-of-function mutation of PBK associated with human kidney stone disease

    doi: 10.1038/s41598-020-66936-4

    Figure Lengend Snippet: Downstream signaling hits between cells expressing wild-type PBK and cells expressing p.Gly43Arg PBK (control and treatment) for 24 hours as analyzed by KAM-900P Antibody Microarray.

    Article Snippet: Protein phosphorylation in HEK293T cells transfected with plasmid construct containing either WT-PBK cDNA or mutant-p.G43R-PBK cDNA was examined by KAM-900P Antibody Microarray Service (Kinexus Bioinformatics Corporation, Vancouver, Canada).

    Techniques: Expressing, Control, Microarray

    Kinexus antibody microarray. Fibroblast growth factor receptor-like protein 1 (FGFRL1) inducible cells (clone K3F) were cultivated for 32 h in the presence or absence of the inducer doxycycline. Protein extracts were prepared and analyzed on Kinexus antibody microarrays containing more than 650 different antibodies against various signaling proteins. No significant differences were observed between doxycycline-treated and untreated cells (not shown). To verify some of the results, the two protein extracts were separated on SDS polyacrylamide gels, transferred to an immunoblot membrane and probed with antibodies against 18 selected proteins as indicated using a multiscreen apparatus from BioRad. The immunoblot was analyzed with a chemiluminescence imager. No significant differences were observed between doxycycline-induced (lower panel) and uninduced (top panel) cells. The migration positions of molecular weight markers are shown in the left and right margins by short horizontal lines. These lines correspond (from top to bottom) to 250 K, 150 K, 100 K, 75 K, 50 K, 37 K, 25 K, 20 K and 15 K.

    Journal: International Journal of Molecular Medicine

    Article Title: Receptor FGFRL1 does not promote cell proliferation but induces cell adhesion

    doi: 10.3892/ijmm.2016.2601

    Figure Lengend Snippet: Kinexus antibody microarray. Fibroblast growth factor receptor-like protein 1 (FGFRL1) inducible cells (clone K3F) were cultivated for 32 h in the presence or absence of the inducer doxycycline. Protein extracts were prepared and analyzed on Kinexus antibody microarrays containing more than 650 different antibodies against various signaling proteins. No significant differences were observed between doxycycline-treated and untreated cells (not shown). To verify some of the results, the two protein extracts were separated on SDS polyacrylamide gels, transferred to an immunoblot membrane and probed with antibodies against 18 selected proteins as indicated using a multiscreen apparatus from BioRad. The immunoblot was analyzed with a chemiluminescence imager. No significant differences were observed between doxycycline-induced (lower panel) and uninduced (top panel) cells. The migration positions of molecular weight markers are shown in the left and right margins by short horizontal lines. These lines correspond (from top to bottom) to 250 K, 150 K, 100 K, 75 K, 50 K, 37 K, 25 K, 20 K and 15 K.

    Article Snippet: The extracts were analyzed by the Kinexus antibody microarray service, which utilizes >650 verified antibodies against most well-studied signaling proteins, including phospholipase Cγ, PI3-kinase, STAT and Akt.

    Techniques: Microarray, Western Blot, Migration, Molecular Weight